ABSTRACT: An experiment was carried out to analyze the effect of sowing dates and genotypic response of sweet sorghum (Sorghum bicolar L.) in relation to plant height, total chlorophyll and nitrogen content in the flag leave at regular intervals i.e. 30, 60, 90 and 120 DAS at the research farm of Lovely Professional University, Punjab. As per the sowing dates is concern, plant height (cm) and amount of chlorophyll (mg g-1 of fresh leaf) and nitrogen content (%) was gradually decreased up to the last sowing dates (D1 to D3). While among the DAS is concern, the amount of chlorophyll content was gradually increased up to 75 DAS and decline thereafter in all the tested genotypes in each dates of sowing. However, the amount of nitrogen (%) was marginally leads by 120 DAS plant as compare to 90 DAS plant. The maximum amount of chlorophyll content was recorded in V1 (SSV 74) followed by V3 (CSV 24 SS) and V2 (SSV 84). While the variety V3 recorded maximum height as well as highest amount of nitrogen content followed by V1 and V2.