Aims & Objectives:Renal biopsy is an essential tool and is the gold standardmethod for the diagnosis of glomerular diseases. It is useful for identifying the specific diagnosis, assessing the level of disease activity, and for allowing specific decisions about treatment to be made.In this study,we tried to examine and interpret renal biopsies using lightmicroscopy, special stains and immunofluorescence, to study the histological pattern of renal diseases on biopsies and correlate clinically Material and Methods: A prospective andreterospectiveanalysis of hundred renal biopsies was done.A minimum of two cores required,one core for Direct immunofluorescence(DIF) in normal saline,another in formalin forLight microscopy(LM) .DIF staining were done withantibodies against Immunoglobulins A, G, M, and C3c, and C1q.In light Microscopy, biopsies were stained routinely with Hematoxylin and Eosin(H&E) and Special stains - Periodic acid schiff(PAS), Masson Trichrome(MT), Silver methenamine(SM) Results:The study included 100 cases.The age distribution among the lesions ranged from 13 years to 72 years.55 cases were males and 45 were females.These included various glomerular and nonglomerulardiseases.Membranoproliferative glomerulonephritis, (n = 17)and tubulointerstitial disease,(n=17 ) being the commonest followed by membranous glomerulopathy (n = 16);diabetic nephropathy (n=11):post infectious glomerulonephritis(n=10); HTN nephropathy(n=8); IGA nephropathy(n=6); Focal segmental glomerulonephritis(n=5); Minimal change disease(n=5);crescentic Glomerulonephritis( n = 3); Lupus nephritis(n=2). Conclusion:1. Renal biopsy is an essential tool in the diagnosis of glomerular diseases. 2. Immunofluorescence plays an important role in isolating and identifying immune deposits in glomerular diseases