Introduction: Drug promotional literatures are one of the important sources of drug information to the physicians provided by pharmaceutical companies through medical representatives. Information provided in the promotional literature should be factual, evidence based, unambiguous and balanced(benefits and risks). Objective: To evaluate the rationality of drug promotional literature using World Health Organization(WHO) guidelines. Material and Methods: This is a Cross-sectional, observational study, conducted at the KBN teaching and general hospital, attached to KBN University-Faculty of Medical Sciences, kalaburagi over a period of 6 months. Drug promotional literature was collected from the out patient departments of KBN teaching and general hospital, kalaburagi. Drug monographs, reminder advertisements, drug lists, ayurvedic medicines, literatures promoting medical devices, equipments or orthopedic prosthesis and literature promoting more than two brands were excluded. Results We analysed 190 drug promotional literatures (DPL). Of which majority of DPL were of antimicrobial agents (20.52%). Other included drugs acting on cardiovascular system (17.36%), anti-diabetic drugs (16.31%), Analgesic agents (8.94%), and least being DPL on drugs acting on Gastrointestinal tract (2.10%). On analysis as per WHO criteria, all DPL mentioned brand names and generic names. More than 75% of DPL presented the beneficial points of the drug like pharmacological effect(93.15%), clinical indication(85.78%), dosage form and strength of the drug(84.21%), dosing interval(79.47%) and less than 15% of DPL presented data on drug safety like adverse effects(4.73%), precautions and warning(13.15%), contraindications (6.31%), drug interactions(11.05%), special situations (2.63%) and overdosage(1.05%). 91.57% of the DPL mentioned Name and address of manufacturer and distributor for correspondence. Reference to scientific literature was quoted in < 50% of DPL(49.47%) indicating the lapse in providing evidence based scientific information.