Spontaneous abortion is one of the most pregnancy problems that happen in early pregnancy when the uterus empties the output of conception. Several factors are associated to causing abortion implicating genetic, endocrinological, immunological and anatomical anomalies as well as infectious agent as infection with herpes simplex virus (HSV-2) is most common virus that widely spread in the world. Objective: the objective of this study to detect the rate of MicroRNA-22 and MicroRNA-184 in the serum of aborted women and compared with pregnant controls. Patients and methods: The current study done on 120 women who include 60 aborted women and 60 nonaborted pregnant women as a control group, the age of both groups are ranged from 15-43 years. The blood samples are obtained from both groups and then centrifuged to obtain the serum that used for detection of HSV-2IgM and other part used for RNA extraction. After extraction of RNA, the relative expression of microRNAs was measured using real-time quantitative reverse transcription-PCR method. Results: The high expression of miRNA-184 was observed in 8(34.8%) aborted women have two or three abortion but only 3(8.1%) of aborted women with one abortion have high miRNA-184. About 23(38.3%) of aborted women with HSV-2 have elevated MicroRNA-22 .Statistically, there was a significant relation between prevalence of MicroRNA-22 and studied groupd (p value=0.005). Conclusion: miR-184 is overexpressed in recurrent spontaneous abortion.