Introduction: Diabetes is characterized by chronic hyperglycemia & disturbances of carbohydrate, lipid and protein metabolism. In subtypes of Diabetes Mellitus, type II diabetes mellitus is the most common endocrine and metabolic disorder. DM is a condition of increased oxidative stress and requires antioxidant. Aim: To assess the association between of oxidative stress and lipid abnormalities in type 2 diabetes mellitus patients in comparison with healthy subjects. Materials & Method: This is a case control study, conducted in Department of Biochemistry, Era’s Lucknow Medical University, Lucknow, U.P., India between January 2022 to June 2022. A total of 90 cases of type II diabetes mellitus & 90 cases of healthy subjects between the age of 30 and 60 years were enrolled in study. Fasting blood glucose, glycated hemoglobin (HbA1c), total cholesterol (TC), triglycerides (TG), high density lipid (HDL) and malondialdehyde (MDA) levels were assessed. Results: The mean age of type II diabetes mellitus cases were higher 55.2±10.1yr and 45±14 yrs in healthy subjects. The body mass index was higher 29.7±3.8 kg/m2 and 24.8±5.3 in healthy controls. Significant difference was observed in FBS, Hb1Ac, TC, TG, LDL, HDL along with VLDL & MDA. With TC, LDL, VLDL, FBS, HbA1c shows positive correlation between MDA and HDL. Conclusion: Increased blood glucose levels along with dyslipidemia in patients with diabetes causes oxidative stress leading to atherosclerosis. Early detection and treatment of lipid abnormalities may be used to minimize risk for atherogenic cardiovascular disorder & cerebrovascular accident in patients with diabetes.