Abstract Background: Herbal mouthwashes carry a wide array of advantages such as antimicrobial properties, antioxidant properties and more. They are affordable and have minimal or no side effects. They are more efficient than commercially available mouthwashes in the market. Objectives: The aim of this study was to compare and to analyze the antimicrobial efficacy of green tea mouthwash, neem mouthwash, homeopathic (Frezyderm) mouthwash, rexidine mouthwash on dental plaque. Materials and Methods: This is a double-blinded, random controlled research study conducted in the Department of Periodontics. A total of 200 patients were selected randomly, comprising of four groups (Group-I:green tea, group-II-:neem sticks, Group-III: homeopathic (Frezyderm) mouthwash and group-IV: rexidine mouthwash) with gingival index score II. Saliva samples were collected on at baseline and on day 10 and 20 and microbial colony count was done. The data obtained was statistically analyzed using SPSS version 26 with ANOVA test. Results: Microbial colonies were reduced better in Group IV on the day 10, whereas on day 20, greater reduction was observed in Group II with the difference was statistically significant.Conclusion: Neem mouthwash formulation had better effects as compared to other three mouthwashes and it could overcome the disadvantages of other mouthwashes as well.