Background: Apical debris extrusion may be clinically associated with pain and/or swelling in the presence of an intense inflammatory response. The present study was to evaluate apical extrusion of debris during endodontic instrumentation with continuous rotation and reciprocating motion. Materials & Methods: 60 extracted single-rooted mandibular premolar human teeth which were divided into 4 groups of 15 teeth each. The canals were instrumented with the following instrument systems: ProTaper Next, 2Shape, Reciproc Blue and WaveOne Gold. Apically extruded debris during instrumentation was collected into pre-weighed Eppendorf tubes. The Eppendorf tubes were then stored in an incubator at 70°C for 5 days. The weight of the dry extruded debris was done with Eppendorf tubes. Results: The mean debris (grams) with 2shape was 0.047, Protaper next was 0.091, Reciproc Blue was 0.054 and WaveOne gold was 0.051. The difference was significant (P<0.05). Statistically significant difference was observed between TS and PTN, PTN and RB and PTN and WOG (P < 0.05).