Eugenol is a phenylpropanoid group compound found in cloves, nutmeg, cinnamon, and bay leaves. Apart from being used as a cosmetic, perfume, and food ingredient, eugenol is known to have an antioxidant, antibacterial, anti-inflammatory, and anti-cancer profile. Eugenol has therapeutic potential by increasing reactive oxygen species formation, decreasing anti-apoptotic protein Bcl-2, increasing the release of cytochrome c that leads to apoptosis in cancer cells, and inhibit the epithelial to mesenchymal transition (EMT) process that could reduce the cell ability to migrating. We synthesized eugenol loaded chitosan nanoparticles (Nano-EU) by ionic gelation method to overcome its shortcoming which is volatile and to increase its bioavailability. The nanoparticles were characterized by using Dynamic Light Scattering (DLS). Anticancer activity of Nano-EU was investigatedin cervical cancer HeLa cell line by flow cytometry using Annexin-V/PI staining, and by measuring cleaved-caspase-3 protein expression which is the executor of the apoptosis process by immunofluorescence. The results of the study evidenced that Nano-EU inducing apoptosis and increasing activated caspase-3 expression in HeLa cells. Nano-EU could also inhibit cell migration by reducing vimentin and Snail as mesenchymal markers leading to inhibition of the EMT process. Further research is still needed to investigate the anticancer potential of Nano-EU in HeLa cells to in vivo and clinical studies.