Aim: Evaluation of OCT 4 expression in oral squamous cell carcinoma is the goal. Materials and methods: The study contained 30 samples of archival tissue blocks, which were then divided into 10 each for the Well-differentiated & Moderately differentiated squamous cell carcinoma in the Cases (Oral squamous cell cancer) groups and 10 blocks for the control normal mucosa. Anti-Oct-4 antibody was used for immunohistochemical staining on the sections of Group I and Group II. The cells were counted, and the data were compared for significance with a p-value of 0.05 being considered significant. Results: When the mean number of positive cells is compared between the control and study groups, statistical significance is seen on the correlation of OCT 4 expressions. When the average number of positive cells in normal mucosa and moderately differentiated Squamous cell carcinoma are compared, a strong statistical significance is seen on the association of OCT 4 expressions. Conclusion: It appears that OCT 4 is involved in determining the prognosis of oral squamous cell carcinoma patients since its quantitative expression rose from normal mucosa to the welldifferentiated variation of the disease, and then to the moderately differentiated variant