Abstract: Background: Urinary tract infection (UTI) is among furthermost spreaded contagion in entire world. Organisms responsible for the infection are varying in their susceptibility pattern in different places and becoming resistant to commonly used antibiotics. Aim: Objective of study was to determine the etiology of community acquired UTI and to determine their antimicrobial susceptibility pattern. Methods and materials: Samples of urine were collected from 120 patients having a clinical diagnosis of UTI. Isolates were recognized by standard biochemical reactions and the antimicrobial susceptibility testing took place by using Kirby Bauer's disc diffusion method. Results: Out of the total sample 65% patients had a positive urine culture. Escherichia coli (42.3%) was most general Gram-negative microorganism & Staphylococcus spp. (15.38%) was most common Gram-positive microorganism. In gram-negative bacilli greater resistance was detected against Amoxyclav and Cephalosporins. Discussion and Conclusion: Study establish that E. coli was the major bacteria causing community acquired UTI in eastern part of Odisha, India. This study verified on growing resistance to Cephalosporins and Amoxyclav among UTI pathogens in the community.