Aim: Therefore, following study was conducted to know the occurrence of candidemia in our region, among suspected septicemic patients. Materials and Methods: This prospective study was conducted between december and april 2017 in the Departments of Microbiology and Pediatrics of tertiary care hospital in central , India. From a total 110 neonates admitted to NICU with clinical suspicion of septicaemia. blood samples were collected in blood culture bottle and incubated at 37C .then subculture done on blood agar and Mc conkey agar and Sabouraud’s dextrose agar with chloramphenicol (0.05%) and incubated at 37C. Only those which yielded pure growth of candida spp. Were included in the study. Preliminary identification was done by colony morphology on SDA , chromogenic medium, growth at 45C , germ tube test , chlamydospore formation and was confirmed by carbohydrate fermentation test. Candidemia was defiend as the presence of at least one positive blood culture containing pure growth of candida spp. With supportive clinical feature.