Antimicrobial barriers are made of polymers of cationic functional groups.PolymerMembrane filtration is considered as highly effective method for removing particles, organic matter, and microorganisms from drinking water.These membranes are usually made from copolymers. In the present work, fabrication of a copolymer membrane wasdone first and a portion of the copolymer's nitrile groups is converted to amidoxime groups through the amidoximation process. After that the mebrane was treated with HA in an aqueous solution at 60-80°C to form an Amidoximated film. These fabricated and specially synthesized copolymer film after the treatment with Nano Silver particles were then studied for its efficacy as antibacterial water disinfectant. Several techniques such as Fourier Transform Infrared (FTIR), Xray diffraction (EDX), Thermogravimetric analysis, Scanning Electron Microscope, and Transmission Electron Microscope were used to characterize the virgin and amidoximated acrylic copolymer membranes. Many significant changes in the copolymer properties, such as functionality, crystallinity, thermal activity, hydrophilicity, elemental composition, and surface morphology, were observed during the amidoximation phase along with their antibacterial activity. It was found that an amidoximated copolymer membrane is more efficient than the untreated membrane in context to its antibacterial activity.