Background: Vitiligo is a chronic skin disorder characterized by patchy loss of skin color and affects approximately 0.5% to 2% of the world’s population. 5-Flurouracil (5‐FU) was tried in treatment of vitiligo in combination with NB-UVB, but was preceded by either dermabrasion or laser ablation of the vitiligo area to ensure more penetration of 5-FU into the skin. The intralesional 5‐FU solution injection is an effective treatment of localized vitiligo and its effects were maintained for 6 months.The aim of the work was to assess the efficacy and safety of intradermal injection of 5-Flurouracil in the treatment of vitiligo.Patients and methods: A total number of 14vitiligopatients were included in the study. They were collected from Outpatient clinic of Dermatology, Venereology and Andrology department, Faculty of Medicine, Zagazig University. All the patients were subjected to complete history taking, complete general examination and clinical examination of vitiligo area. Then, intradermal injectionof 5-Flurouracil every 2 weeks for 3 months was done. Any complications were recorded andpatients were photographed before and after treatment to assess the clinical repigmentations.Results:Efficacy of the treatment was 78.6% but the efficacy of treatment of acral type was the lowest among vitiligo cases.Conclusion:Intradermal injection of 5FU is safe and effective inthe treatment of vitiligo