Background: Securing intravenous access in children is often challenging for health care professionals. Children view needle sticks as the worst source of pain and fear in the hospital setting. We have undertaken this study with the aim of evaluating the efficacy of EMLA cream in reducing pain while securing intravenous cannula in paediatric patients. Methods: Fifteen pediatric patients between age group of 2 year to 12 years requiring intravenous access for medication purpose prior to surgery were selected and eutectic mixture of Prilocaine 25mg and Lidocaine 25 mg (Prilox Cream) was applied on desired hand and an occlusive dressing was applied to protect the area. After completion of 60 minutes dressing was removed and intravenous access was attempted. Children rated any pain associated. Results: 11 (73.33%) patients did not complain of pain, 3 (20%) had mild (1-3) pain scores & 1 (6.67%) was having moderate (4-6) pain score. In this study none of the patient was having severe (7-9) or worst (10) pain. Pain ratings and behavioral distress ratings were generally in the low to moderate range for all patients. Conclusion: EMLA cream (Prilox cream- Lignocaine and Prilocaine) is effective for producing topical anesthesia for i.v. insertion in paediatric patients.