Abstract Background : A serious threat to both general and medical community is the proper collection and proper dumping of the Bio-medical Waste. In India, 1.87 lakh healthcare facilities with 17.01 lakh beds generates 519 tonnes of biomedical waste every day, Central Pollution Control Board (CPCB). There are Healthcare Facilities (HCF) that still handle and accumulate such wastes inappropriately without understanding its hazardous repercussions on environment and human society. Study is attributed to awareness and negligence related issues. It covers a wide spectrum of information required for Healthcare Facilities (HCF) in regards to modifications in Bio-Medical Waste Management Rules, 2016’ (BMWM2016) from year 2016 till November 2019 and rules amended in view of the COVID-19 pandemic. Aim : Effectiveness of Training Program on awareness among Nursing Staff, Lab Technicians and Sanitary Workers regarding Bio-medical Waste management at a Tertiary Care Hospital in Central India. Methodology : Interventional Study will be implicated from September 2020 to December 2022 in Acharya Vinoba Bhave Rural Hospital (AVBRH) Tertiary Care Hospital, Sawangi, Wardha, Central India by the Post Graduate Student. Assessment of awareness will be done by distributing a set of questionnaires and then training the Healthcare Personnel (HCP), regarding Bio-Medical Waste Management based upon direct observation in the workplace. A total of 422 Nurses, Lab Technicians and Sanitary Workers will be enrolled as study participants. The Study participants will be demonstrated regarding proper standards of Bio-Medical Waste management. Expected Outcomes/Results and Conclusion : Data will be analyzed and appropriate statistical tests will contemplate the results of study.