Objective: The objective of the study is to evaluate the effectiveness and tissue response of intralesional platelet‑ rich plasma (PRP) injection with serial USG follow up among 40 patients in plantar fascitis. Materials and Methods: A total number of 40 patients with plantar fascitis. Pre‑ and post‑ intervention visual analogue scale (VAS) for the assessment of pain relief and assessment of plantar fasicia(PF) thickness using USG with 1 month, 3 month and 6 month follow up. Results: The mean VAS scores for heel pain measured at 1st month of treatment was 3.025±0.831, at 3months was 2.225±0.5767, at 6months was 0.025±0.1581. The decrease in mean VAS score was statistically significant when compared with pre-treatment values (8.926±0.565). The mean plantar fascial thickness measured at 1 month was 3.575±0.5006, at 3 months was 3.4±0.4961, at 6 months was 3.1±0.2819. The decrease in mean plantar fascial thickness was statistically significant when compared with pre-treatment values (6.5725±0.7161). Conclusion: Intralesional injection of the PRP is effective and safe modalities of treatment for plantar fasciitis with significant reduction in PF thickness.