Abstract: This study aimed to determine the effect of various doses of FSH on the superovulation response of swamp buffalo. The experimental subjects used were 16 swamp buffaloes with no pregnant status, 3-5 years of age, a calf, normal estrous cycles, and clinically healthy reproduction. The swamp buffaloes were made to have synchronized estrous with double dose injection of FSH and PMSG hormones and were injected intramuscularly using four FSH doses as a treatment: 320 mg/16 ml, 360 mg/18 ml, 400 mg/20 ml, and 440 mg/22 ml. The experimental design used was CRD (Completely Randomized Design), which consisted of four replications. Parameters observed included: superovulation response, number of corpus luteum, onset, and estrous in swamp buffalo. The results obtained were superovulation response of up to 56.25% of the swamp buffaloes. The average number of corpus luteum, onset, and estrous in the swamp buffaloes were 2.75, 27.75, and 32.50 h, respectively. It was concluded that superovulation with doses of 320 mg/16 ml, 360 mg/18 ml, 400 mg/20 ml, and 440 mg/22 ml FSH showed positive responses, but 320 mg/16 ml as an optimum dose produced more corpus luteum.