Abstract Background: Diabetes puts people at a higher risk for a wide range of microvascular and macrovascular problems. Aim: The aim of the present was to study the effect of hyperglycemia, HbA1c on total cholesterol (TC) and High-Density Lipoprotein (HDLc) in Type 2 Diabetes mellitus (T2DM) subjects. Materials &methods: In this case-control study, we compared a group of people with type 2 diabetes (T2DM) with a group of people without T2DM, totaling 200 people. All participants were evaluated at Index Medical College & Research Centre in Indore, India, using the center's outpatient services. The authors of the study have begun their work after receiving approval from the relevant ethics board. Each individual in this study has given their consent before the study began. Results: Age, BMI, FBS, and HbA1c, were measured. BMI, FBS (t=6.955, d=198), HbA1c (t=10.931, d=198), shown to be significantly higher in T2DM patients compared with healthy controls. This shows that increased glucose levels may hamper the sensitivity of the insulin and as well as glycation of the proteins. On the contrary, the present study did not observe significant difference in case with the age of the subjects present in the study. TC, and HDLc, levels were shown above. TC (t=5.043, d=198) level was significantly higher in T2DM patients compared with healthy controls. Conclusion: Reducing ROS generation may lower hyperglycemia, dyslipidemia, and oxidative stress. These advancements may result from: However, lowering oxidative stress, which causes hyperglycemia and dyslipidemia, may increase serum insulin levels, according to the study's authors.