Aim: To evaluate the effect of eccentric wrist extensor exercises in patients with chronic lateral epicondylitis. Material and methods: The present prospective observational study was conducted in the department of orthopaedics among 100 patients, who come to our institution with complaints of pain and tenderness on palpation over the lateral epicondyle of humerus. Patients assigned to Group received supervised therapeutic exercise program which include eccentric strengthening of the wrist extensors. Patients were provided with education manual regarding ergonomics and activity modification technique to avoid aggravation of symptoms. Patients were followed up at subsequent visits i.e. at 2 weeks, 6 weeks and 3 months and TEFS (Tennis Elbow Functional Score) and VAS for pain scores were documented at the time of presentation and at subsequent visits. Results: Previous episode of lateral epicondylitis was reported among 25% of the subjects. Duration of present episode was reported by 60%, 28% and 12% of the patients since 1-5, 6-10 and >10 weeks respectively. Mean VAS score of pain at baseline, 2 weeks, 6 weeks and 3 months was 6.35, 4.31, 3.14 and 2.16 respectively with statistically significant difference when compared using anova test as p<0.05. It was found that TEFS score at all the intervals was decreasing.