The use of medicinal plants in treatment has been very common nowdays. Colocynth, Scientifically kwon as Citrullus colocynthis is used to reduce pain and inflammation in traditional medicine. C. colocynthis are already reported as rich source of different phytochemical constituents and responsible for many pharmacological applications. The aim of present study is to evaluate the effects of acetone extract of Colocynth fruit (AECC) on pain and inflammatory mediators at doses of 100 mg/kg, 200mg/kg, 400mg/kg by using tail flick and formaline induced pain methods. Our observations indicated that C. colocynthis fruit acetone extract can reduce pain and inflammation and we can use this plant as analgesic and anti inflammatory agent in the future.