Abstract: Diabetes mellitus is characterized by loss of glucose homeostasis as a result of impaired secretion and function of insulin. The present study was designed to evaluate the role of flower extractof Bergenialigulataagainst diabetesin Streptozotocin induced diabetic rats. Albino Wistar rats weighing 120-150g of either sex were selected for the study.After a week of acclimatization, the rats were subjected to overnight fasting. Diabeteswas induced by intraperitoneal injection of Streptozotocin, freshly dissolved in citrate bufferpH 4.5.In the present study the STZ control animals showed enhanced gluconeogenic enzymes and diminished glycolytic enzymes there by it disturb the glucose homeostasis and diminished insulin production leads increased serum glucose levels. Treatment with flower extract ofBergenialigulata(AFBL) showed significant balancing of glucose homeostasis through reduction in gluconeogenic enzymes and elevation of glycolytic enzymes.The selected flower extract ofBergenialigulata(AFBL) showed in vivo antioxidant activity, antihyperglycemic activity, hepatoprotective activity, nephroprotective activity and finally glucose homeostatic activity might be due to the potent phytoconstituents present in the selected flower extract.