Abstract: Background: Labor is one of the most important events in every women’s life. A healthy mother and a healthy baby without complication is everyone’s demand. Women experience many discomforts during labor and childbirth. Pregnant women commonly worry about pain, duration and process of labor. Objective: To assess and compare the effect of ambulation on labor outcomes during the first stage of labor. Methods: A quantitative research approach with only a posttest control group design was taken to conduct the study. A total of 60 Prime mothers having 37-42 weeks of gestation with single vertex presentation and spontaneous onset of labor in 1-2 centimeters of cervical dilatation (latent phase) were taken by purposive sampling technique. The test group was assigned to walk for two hours during the first stage of labor. The post-test was followed by assessing the progress of labour with the help of a Structured Observational checklist. Results: The result of the study shows that mean & SD 17.16+ 2.8 on labor outcome in the experiment group and the mean + SD (13.5 + 4.3) in the control group was statistically significant as evident from t = 3.91 at df 58 and p-value was 0.0001. It shows that ambulation has positive effects on labour outcomes. Conclusion: Hence it was evident that ambulation during the first stage was effective in improving labor outcome, helps in early descent of the fetus, increases coordination of uterine contraction intensity and frequency among prime mothers. Based on the study findings, the recommendations were made for a study on a large sample and different settings.