1. Introduction: The virus that causes COVID-19 diseases is Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), has generated pandemic which requires preventive measures, vaccines and pharmacological interventions. Some studies showed effectiveness of Chloroquine and Hydroxychloroquine for the treatment and prophylaxis.HCQ acts mainly by modulating cellular metabolism of coronaviruses. Due to cost-effectiveness and availability ICMR also recommended its use for prophylaxis and found effective. So, this study was carried out to document perceived effectiveness and adverse effects of HCQ as prophylaxis. 2. Methodology: A cross-sectional e-survey was carried after ethical approval and validated questionnaire had been sent to 500 health care professionals via google form online.In 3 months of duration, 193 responses were received (whohad taken HCQ prophylaxis), data collected and analyzed. 3. Results: Among 193 responses, 109from government and 84 were from private set up. The most common reason(s) to take prophylaxis was direct contact with positive cases, selected by 94 (50.5%) responders and 133 (70%) had completed the course as per ICMR guideline. 86.5% participants didn’t have infection while others were positive cases and among them 69% had mild to moderate symptoms. Nauseawas most commonadverse effects followed by dizziness, vomiting, abdominal pain, hypoglycemia, hypersensitivity, etc. No any cardiovascular effect was noticed and 62.8% agreed to recommend HCQ as prophylaxis to others. 4. Statistical analysis: Data analyzed using descriptive statistics with SPSS 1.0.0.1406 version 5. Conclusion: HCQ prophylaxis against COVID-19 among health care professionals showed perceived effectiveness without any serious adverse effects here which is shown by other studies also and so it is considered as complementary strategies for prophylaxis with vaccinatio