Abstract : Our finding may improve the understanding of Dynamic warm-up protocol with and without weighted vest on fitness performance and may help clinicians develop effective warm-up protocols for sports practice and competition. This paper aims to investigate the effect of dynamic warm-up protocol with and without weighted vest on fitness performance in varsity female athlete. A total of 40 female university athletes were recruited voluntarily. After 5 minutes of jogging, subjects performed dynamic warm-up protocols: (1) Nine moderate-intensity to high-intensity dynamic exercises without weighted vest (DY), (2) the same nine dynamic exercises performed with a vest weighted with 6% of body weight weighted vest (DY6) and (3) the same nine dynamic exercises performed with a vest weighted with 12% of body mass (DY12). The main outcome measures are vertical jump (VJ), standing broad jump jump (SBJ), seated medicine the ball toss (SMBJ), and 20 meter sprint (S). The result showed that VJ performance was significantly greater after weighted vest with DY6 and DY12 (39.93±1.06cm, 40.68±0.94cm, respectively), compared with DY (37.63±1.00cm), and SBJ performance was significantly greater after DY6 and DY12 (65.00±9.40 inch, 66.30±8.41inch) compared with DY and DY6 (62.80±8.70 inch) (P<.05). SMBT with DY12 had been increase to 2.80±0.27m from DY and DY6 (2.55±0.30m vs 2.70±0.29m, respectively) and 20m sprint with DY12 weighted vest had been reduced to 3.75±0.29sec while 20m sprint from DY with DY6 weighted vest (3.95±0.85sec vs 3.78±0.28sec, respectively). Therefore dynamic warm-up performed with a vest weighted with 6% and 12% of body mass may be the most effective warm up protocol for enhancing jump performance in varsity female athlete.