Abstract: This study aims to analyze the influence of Gemba Kaizen culture and implementation of 5S (Seiri, Seiton, Seiso, Seiketsu, and Shitsuke) to Medical Equipment manufacturing performance in the Medical Equipment manufacturing industries in Indonesia. This research uses a quantitative approach with Structural Equation Modeling (SEM) using smart PLS software. Data collection methods using online questionnaires and simple random sampling technique, the number of samples to be used as respondents as many as 300 Medical Equipment manufacturing managers. The results of this study indicate that the gemba kaizen has a positive and significant effect on the Medical Equipment manufacturing performance. For 5S also has a positive and significant effect on on Medical Equipment manufacturing performance. The implication of this research is to be a reference that kaizen and 5S can be applied in several manufacturers to improve their performance and can be a reference to be applied in other industries . This novelty of this research is build a new model of the effect of applying new kaizen and 5S to the Medical Equipment manufacturing industries in Indonesia.