In all the cells of the human body, the fundamentally existing genetic material is Deoxyribonucleic Acid (DNA). The DNA of every single person varies from one another. In criminal assessment, the DNA evaluation has been utilized as vital evidence. It is also employed in cases concerning the ascertainment of Paternity of Identity in civil proceedings. The court constantly depends upon the accurateness, lawful accumulation, protection, along with certification of the DNA evidence’s admissibility. The court is convinced of this evidence because of its trustworthiness. An act done by an individual without criminal intention is not termed a crime. In civil along with criminal cases, the DNA assessment has turned into a highly significant task. Conversely, to support investigators together with courts in cases including DNA evidence, no particular law is available in India. Thus, a person is alleged or removed from doubt with the DNA evidence as collected from the scene of Crime. Several methodologies utilized for the recognition of DNA evidence are surveyed in this work. There would be remains of biological proofs like skin cells, hair, blood, or semen available on victim’s body at the scene of the Crime. The collection and processing of such biological samples for gathering DNA Evidence is a complex and has to be meet certain legal standards to be admissible. The aim of this paper to make a review of the evidentiary value of DNA as collected and processed from the Crime scene