Abstract: The harmful amounts of metals in soils may be related to natural vegetation due to planting, manufacturing, mining, and waste management activities. Many of the acidic soils below pH 5.0 are essential growth restricting factors in plants, pH value as large as 5.5, however, may be easily reached. The issue is especially bad in extremely acidic subsoils that face trouble during liming and are aggravated by strongly acidic nitrogen engravings. The strong acidity in the subsoil (AI toxicity) decreases height of the plants, increases drought susceptibility and decreases the use of subsoil nutrients. The high temperature exacerbates aluminum toxicity d in cotton and wheat. The treatments T4, when compared with T1, showed that Fibroin NPs decreased the total free proline in rice stalk by only 14.19% whereas KNO3 NPS in T5 enhanced the same by 39.92% when applied along with Aluminium stress. The total free proline in rice stalk was significantly enhanced by about 9.88% concerning T1 when treated with Fibroin NPs upon Aluminium stress whereas only sole Fibroin NPs were applied (T6). KNO3 Nanoparticles when applied upon Aluminium stress (T6). The total free proline in rice roots was significantly decreased by about 17.39% concerning T1 when treated with Fibroin NPs upon Aluminium stress whereas only sole Fibroin NPs were applied (T6). KNO3 Nanoparticles when applied upon Aluminium stress (T6).