Abstract: A field experiment was carried out to study the effect of different fertilizer doses and bio-fertilizer inoculation on nutrient content and uptake of chickpea (Cicer arietinum L.). Complete Randomized Block Design with 3 replications and 7 treatments was used for the study and the experiment was conducted during rabi season of 2017-2018 in the experimental farm of Lovely Professional University, Phagwara, Punjab. Application of 100 % RDF (T1) and 75% RDF + PSB as soil application + Rhizobium as seed treatment (T2) showed remarkable results on seed and stover yield; nutrient content and uptake by seed and stover; and protein content of the seed. Significantly higher seed yield (1348.75 kg ha-1) and stover yield (2370.37 kg ha-1) of chickpea was observed on application of 75% RDF + PSB as soil application + Rhizobium as seed treatment (T2) and 100% RDF (T1) respectively. Higher nutrient content in seed and stover, higher nutrient uptake by stover and higher protein content in seed was recorded with the application of 100% RDF which remained at par with T2. Interestingly, higher nutrient uptake by chickpea seed was revealed with the application of 75% RDF + PSB as soil application + Rhizobium as seed treatment and it remained at par with T1.