Abstract: This research purpose to analyze the effect of implementation of 5S (Seiri, Seiton, Seiso, Seiketsu, and Shitsuke) to business performance in the medical health industry industries. This research uses a quantitative approach with Structural Equation Modeling (SEM) using smart PLS software. Data collection methods using online questionnaires and snowball sampling technique, the number of respondents to be used are 240 respondents of medical health industry managers in Indonesia . The results of this research conclude that Seiri has a positive and significant effect on the business performance, Seiton has a positive and significant effect on the business performance, Seiso has a positive and significant effect on the business performance, Seiketsu has a positive and significant effect on the business performance, Shitsuke has a positive and significant effect on the business performance. This novelty of this research is build a new model of the effect of applying Seiri, Seiton, Seiso, Seiketsu, and Shitsuke to the medical health industry industries in Indonesia.