The current study was conducted for the period from November 2021 until May 2022, which aims to study an epidemiological and diagnostic study of the parasite Entamoeba histolytica and to measure some immunological parameters of those infected with the parasite. The results of the microscopic examination of 1933 samples infested with 327 samples of tissue-forming amoeba parasites, with an infection rate of 17%. In the month of October, it was 8%, and the results showed that the highest percentage of infection with the tissue-dystrophic amoeba parasite was in the liquid stool samples, which amounted to 68.6%, respectively, and the lowest percentage was in the solid stool samples, which amounted to 2.4%. Males are higher than females, reaching 53.5% and 46.5%, respectively. The results also showed that the highest percentage of amoeba parasites was for the age group 21-30 years, which amounted to 23.9%, and the lowest percentage for group >50 reached 11.6%. The ELISA technique increased the concentration of interleukin-2 in serum for those infected with the histiocytic amoeba parasite, as the ratio reached 61.4 ng/ml compared with the serum group of healthy people, which amounted to 4.1 ng/ml. The results showed high concentrations of TNF-a and anti-IgE in serum for those infected with E. Histolytica parasite, it reached (28.7 and 29.3) ng/ml, respectively, compared to the healthy serum, where the concentrations of TNF-a and IgE antibody reached 25.3 ng/ml.