Helicobacter pylori (H.pylori) is a bacterial species that resides in a specific region of the stomach lining known as the gastric antrum. It exists in a layer between the outer surface and slimy layer on top of the inner lining, where the level of acidity is around seven.The present study was conducted among 170 patients at Krishna Hospital and Medical Research Centre, Karad with symptoms suggestive of Acid Peptic Disease (APD) and Dyspepsia. In this study, majority of the patients, i.e., 123 (72.4%) diagnosed with the symptoms suggestive of APD out of which 98 (79.6%) H. pylori infections were reported to be positive on Histopathology, and 96 of them were correctly identified by Rapid Urease Test (RUT). The remaining 47 (27.6%) patients presented with symptoms suggestive of dyspepsia out of which 34 (72.34%) came positive on Histopathology and all of them were correctly diagnosed by RUT. Also 69 (40.6%) patients had features suggestive of diffuse gastritis out of which 59 (85.5%) were positive for disease on Histopathology, out of which 58 of them were correctly diagnosed by RUT. And 15 (8.8%) patients had features of antral gastritis out of which 12 (80%) had the disease on Histopathology. Statistical analysis of the data was performed on SPSS and MS Excel 2019.Furthermore, sensitivity of RUT to correctly diagnose patients suffering from H pylori infection was 90.91%, and Specificity of the test for predictive value for the same was 73.68% with a Positive (PPV) of 92.31% as well as Negative(NPV) of 70% and Diagnostic accuracy of it was 87.06%.