Identification of alkaloidic substances of on Citrullus colocynthis Fruit done by using High Performance Lquid Chromotography(HPLC) technique,the results appeared the fruit containe many compounds as:Citrullo,Cucurbitacin,Colocyanthin and Colocynthitin by 17.68,39.84,29.67 and12.80%.Isolation and Identification of bacteria done at College for women laboratories/Tikrit University, As it was collected (200) median diuresis samples from the reviewers and sleepers in Salah Al-Din General Hospital / Tikrit city / Iraq with urinary tract infections and it included males and females for ages between (15-60) years, The results showed (160) growth samples when cultivated, at a rate of (80%), as they gave two types of growth, negative for the gram stain (65.6) and positive for the gram stain (34.4). The infection rate was higher in females (66.87%) while males (33.13). The susceptibility of the isolated bacterial species to resistance to 6 antibiotics was tested for different groups, in which the bacterial isolates differed in their sensitivity and resistance to the studied antibiotics, The effectiveness of aqueous and alcoholic extracts was also tested at concentrations of 25%, 75% and 100%, where the alcoholic extract was more efficient compared to the aqueous extract, and the concentration of 100% was the most effective concentration on the bacterial isolates..