Abstract. Introduction: Adolescence is the second most critical period of physical growth in the life cycle after the first year. The increased prevalence of unhealthy lifestyles among adolescent girls has become a public concern, as they should maintain their health status for the health of their future children. Aims: This study aims to analyze the determinants of healthy lifestyle among adolescent girls based on Pender’s Health Promotion Model. Method: This was a descriptive-analytic study with cross-sectional approach. The population was adolescent girls (15-18 years old) at Senior High School, Gresik City, East Java. Samples were 210 respondents, taken by using simple random sampling technique. The independent variables were intrinsic motivation, perceived benefits, perceived barriers, self-efficacy, and peers support. The dependent variable was healthy lifestyle behavior. Data were collected by using questionnaire, then analyzed using chi-square (x2 ) (α <0.05). Result: Result showed significant correlation between intrinsic motivation (p=0.001; C=0.253), perceived benefits (p=0.001; C=0.246), perceived barriers (p=0.002; C=0.233), self-efficacy (p=0,000; C=0.436), and peers support (p=0,000; C=0.348) with adolescent girls’ healthy lifestyle. Conclusion: Positive cognition on benefits, no barrier perceived, strong self-efficacy, and strong peer supports would increase adolescent girls’ healthy life.