Abstract: The sprayer is a system capable of turning liquid into mist or solution. Based on spraying efficiency and spray field efficiency, sprayer performance can be evaluated. Parameters of spraying efficacy are in the form of droplet diameter and percentage of dead weeds, whereas efficiency of spraying field parameters are in the form of effective field capacity and theoretical field capacity. This research seeks to develop a bicycle-powered sprayer and, when used by farmers, to measure quantitative workload and qualitative workload. Workload analysis has been carried out with the heart rate parameter on the use of the knapsack sprayer, and both qualitative and quantitative workload will be considered. As observed participants, four males and four females were questioned. The Heart Rate Monitor (HRM) was used to record the heart rate during the operations. Previously, step test calibration was carried out for each subject in order to obtain individual HR-Workload correlations. The result shows that women have a higher qualitative workload than men, although there is no real difference in the quantitative workload for women and men. Subjects' work performance was also determined based on field capability and consumption of srayer fluid. The result showed that the energy consumption rate of women per hectare of work and per tank (20 liters) of spraying is much higher than that of men. Spraying activity using a knapsack sprayer is more appropriate for men (as a male job) compared to that for women, based on the results of the study.