BACKGROUND: Dermatoglyphic pattern, epidermal ridges, triradii develops between 4th & 11th week of intrauterine life persists all through the life. Estimation of palmar angle bilaterally among varying gender may provide details for forensic experts, researchers to correlate with existing diseases in the community serving as a screening tool. AIM: To analyze the dermatoglyphic pattern quantitatively using palmar angles among varying gender bilaterally in South Indian population and identify its significance. MATERIALS AND METHODS: The present study was undertaken with an aim to analyze the dermatoglyphic features in both the gender. The study consists of 200 participants consisting of 105 males and 95 females. Dermatoglyphic prints were taken by “Ink Method” described by Cummins and Midlo and further subjected to statistical analysis to find the varying angles. RESULTS: There was a significance value noted with left ATD (P < 0.001) when compared to right. There was no significant value bilaterally found with varying gender with respect to the remaining palmar angles. CONCLUSION: From the present study, it appears that there exists variations in the palmar angle with varying side, which can be used as a screening tool for determining quantitatively, correlating with various congenital diseases due its genetic influence.