Pharmaceutical adjuvants known as cyclodextrins are widely used. These adjuvants' chemical structure can interact with drug molecules of the proper size to produce an inclusion complex. The main goal of the previous development of such complexes was to make drug more soluble in water. Instead, the focus of the current study is on enhancing the permeability of guest molecule from BCS class III, and the solubility of the complex was also examined as a result of using poloxamer (Pluronic F 108). Method-Acyclovir was chosen as the guest molecule and Hydroxypropyl Beta Cyclodextrin (HPβ-CD) and Randomly Methylated Beta Cyclodextrin (RMβ-CD) as the host molecules, respectively, for the production of host-guest inclusion complexes. Pluronic F 108 was taken as a surfactant. Result-The absorption and scattering properties of prepared complexes were analyzed by UV Spectroscopy and it was found that the rate of agglomeration of complexes was directly proportional to the concentration of Cyclodextrin added which concluded that the permeability of the drug enhanced on complexation with cyclodextrin and further enhancement was observed with addition of Pluronic F 108.