Purpose: Diagnosis of acute undifferentiated febrile illness (AUFI) has been a challenge and burden in clinical practice in the tropics. COVID-19 cases that present with fever alone may be difficult to distinguish from other AUFIs in the tropics. Malaria, Scrub Typhus and Dengue fever are among the most common endemic diseases in the tropics. With the availability of rapid sero-diagnostic tests for these infections, it has been observed that patient’s samples frequently show seropositivity for two or more infections posing challenges in clinical diagnosis and treatment. This study was performed to determine the false-positive serological test (seropositivity) in COVID-19 patients for Scrub typhus, Dengue and Malaria.