Background:- Agni is an important factor for digestion and metabolism in our body. Ingested food is to be digested, absorbed and assimilated, which is unavoidable for the maintenance of life and is performed by Agni. In Ayurveda, the term “Agni” is used as a key factor responsible for digestion of food and metabolic products Objectives are to study the correlation of “Agni” with serum bile acids and sama-Nirama mala Methodology:-Random Selection of clinically normal Subjects followed byassessment of Agni, Prakruti and Koshtha based on Subjective parameters, Observation of Sam - Niram quality of Malaalso estimation of bile acids in blood of all the selected participants, Correlation of Subjective and objective parameters related to Agni and bile acids. Results:- will be drawn from the observations of data Conclusion:- will be drawn from observations, results and statistical analysis of the study.