ABSTRACT : Aim: The purpose of our research was to assess the prognosis of the treatment performed using second generation platelet concentrates (PRF) and a new calcium silicate-based material (Biodentine) for coronal pulpotomy techniques. Methodology: Pulpotomy procedure was carried out on 4 carious involved, permanent molars detected with acute irreversible pulpitis in 17- to 22-year-old patients. Taking into consideration the patient’s age and the condition of the underlying pulp tissue, PRF pulpotomy was planned in view of preserving the vitality of the intact radicular pulps. Regenerative procedures with second generation blood matrices were chosen to encourage the recovery of the inflamed pulps. The follow-up examinations were conducted at 6, 12 and 24 months, revealing a better clinical and radiological result. Descriptive analysis was carried out to assess the prognosis all these teeth with subsequent follow ups. Results: The clinical follow-up evaluation of the cases was met with a positive outcome. In addition, the digital radiographic examination (RVG) of the cases revealed an intact PDL space and a normal trabecular pattern of the bone. There was statistically significant dentinal barrier formation, which shows the healing reponse. Conclusion: We can conclude that clinicians can safely rely upon advanced non-invasive, regenerative approaches to improve the standard of care delivered to the patients and can be considered as an alternative to conventional RCT.