Rate of Vancomycin resistance among Enterococci is increasing. This study was conducted to know the rate of vancomycin resistant enterococci and to compare the sensitivity and resistance of enterococci to vancomycin and to determine the minimum inhibitory concentration of both vancomycin sensitive and vancomycin resistance enteroccci. Materials And Method: In the current study, total 53 urinary isolates and 7 pus isolate of enterococcci strains were identified. The antibiotic susceptibility testing was determined for vancomycin using KirbyBauer disc diffusion and E-Strip test method. The interpretation was done according to CLSI guidelines and the results were compared with Vitek2. RESULTS: Out of 60 isolates, 58.3% of the isolates were identified as E. faecalis and 41.6% of the isolates were identified as E. faecium. 54 enterococcal isolates were sensitive to vancomycin and 6 isolates were vancomycin resistant. High level resistnce of enterococcal islotates towards tetracycin, ciprofloxacin and levofloxacin were observed. More numbers of enterococcal islotaes was observed in males than females. The enterococcal infections were more in in-patients, the infections caused by out-patients is very less. CONCLUSIONS: Our study showed that the results obtained from disc diffusion and Estrip test were same. Discrepancy was observed when the results were compared to vitek results. By this we concluded that disc diffusion and E-Strip method can be widely used methods at peripheral laboratories where automated systems not available.