Abstract Aim: To compare macular and peripapillary RNFL thickness in amblyopic & fellow eye in unilateral amblyopia, using OPKOS SLO/OCT. Material & Methods: This was an Institutional prospective analytical study conducted over a period of one year, after approval from Scientific and Ethical Committee. All the unilateral amblyopic 5 to 40 years of age, who gave consent for undergoing OCT were included in the study. Results: During the study period, 100 eyes of 50 patients, who met the inclusion criteria were taken up for the study. Out of 50 patients, 30 were males and 20 were females with mean age of 20.7 ± 8.9 years. Patients were subdivided into types of amblyopia and anisometropia. The Mean Macular Thickness of amblyopic eye (273.61 ± 20.30) was statistically significantly increased in 50 patients (P=0.05) when compared to fellow non-amblyopic eye (262.81 ± 17.75). Conclusion: There was a significant increase in mean macular thickness in all amblyopes, which was significant only in anisometropes, while RNFL thickness was significantly increased only in strabismic amblyopia, when compared with fellow eye. These results corroborate with findings of similar previous studies.