Introduction: Tuberculosis is an infection caused by a slender shaped, non-sporeforming aerobic acid fast bacilli named -Mycobacterium tuberculosis. Lymph node tuberculosis constitutes 20-40% of extrapulmonary tuberculosis. It is more common in developing and underdeveloped countries. Materials & Methods: A total of 76 patients were enrolled in this study who were having a non-malignant lymphadenopathy more than 1cm above 10 years of age were included in this study. The material obtained from FNAC was divided into three parts. One part was used to prepare a dry fix smear for Giemsa staining for cytology examination. Second part was used to prepare ZN staining and remaining part was used for molecular detection of mycobacterium tuberculosis by RTPCR. Results: A total of 76 patients were enrolled in this study. Out of 76 cases 52 (68.42%) were male while 24 (31.58%) were females and mean age group was 31-40 yrs was found. On FNAC staining tubercular bacilli was found only in 27 (35.5%) cases while on RT-PCR 48 (63.15%) cases were found positive for M. tuberculosis. RT-PCR showed sensitivity of 100%, specificity of 96.55%. Positive predictive value of 97.92%, negative predictive value of 100% and an accuracy of 98.68%. Conclusion: For extra pulmonary lymphadenopathy cases molecular methods are more reliable and sensitive method in a comparison of cytological microscopic methods. Molecular detection required extensive lab setup but it would be a great tool along with a combination of cytological method for the accurate diagnosis of tubercular lymphadenitis.