Background: Combination guided tissue regeneration for the treatment of periodontal intrabony defects is an accepted treatment modality for many years and has undergone a number of changes. Human amniotic membrane allografts have been used for a variety of reconstructive surgical procedures since the early 1900s. The purpose of this study was to clinically and radiographically evaluate the efficacy of amniotic membrane as a guided tissue regenerative material in the treatment of human periodontal intrabony defects.