Background: To compare absorbable with non- absorbable sutures in closure of laparotomy incisions. Materials and Methods: Sixty patients of both genders were divided into 2 groups of 30 each. In group I, fascia was closed with Prolene and in group II, fascia was closed with Vicryl. Using continuous suturing technique, fascia was closed with same size suture in both groups. Length of suture in both groups was constant 4:1. Post-operative infection at 3rd, 5th, 7th and 9th days was recorded. Results: Group I had 15 males and 15 females and group II had 16 males and 14 females. Procedure was elective performed in 17 in group I and 18 in group II and emergency 13 in group I and 12 in group II. Diagnosis was hemoperitoneum 11 in group I and 12 in group II, blunt trauma abdomen 6 in group I and 3 in group II, gut gangrene 3 in group I and 2 in group II, mass abdomen 2 in group I and 3 in group II, intestinal perforation seen in 5 in group I and 6 in group II and intestinal obstruction 3 in group I and 4 in group II. The difference was significant (P< 0.05). Wound dehiscence was seen in 7 in group I and 12 in group II. The difference was significant (P< 0.05). Conclusion: Prolene as compared to absorbable Vicryl suture had less wound dehiscence and better outcome.