Aim: Comparative study on the effect of VIT-D supplementation onthe treatment course of pulmonary tuberculosis. Material and methods: Patients presenting with sign and symptoms suggestive of pulmonary TB, diagnosed as TB and who were receiving the treatment from the study institute. TB Score, Smear Conversion, Health Related Quality of Life, Changes in the level of C-Reactive Protein, Erythrocyte Sedimentation Rate (ESR), and Haemoglobin concentration and Serum Vitamin D levels were measured. At the beginning of the treatment patients were divided in the following two groups based on the mutual decision of the treating physician and the participants. 100 patients were include in this study and divided into two equal groups. Supplementation Group: Patients advised category I DOTS therapy and Vitamin D supplementation. Routine Care Group: Patients advised category I DOTS therapy. Results: The mean time to sputum conversion was shorter among patients given Vitamin- D (9.7 weeks) in comparison to control group (11.2 weeks), however, the difference was not statistically significant (p=0.0621). After being on treatment for 8 weeks; the TB score decreased to 4.90 in the Vit-D group and 6.6 in control group, the difference was statistically significant. (p-0.0012). However, there was no statistically significant difference in the TB score at 12 weeks of treatment (p=0.342). After taking the treatment for 8 weeks, the quality-of-life score was 15.1 in Vit D group and in control it was 13.4, the difference is statistically significant (p-0.004). At 12 weeks also the difference in QoL score was significant (p<0.001) among the two groups. Consequently, the overall change in Quality-of-life score was also statistically significant. (p<0.001). The change in Serum VitD values was almost similar between both the test and control groups at 0 weeks (42.979 and 40.788 respectively) and the difference is not statistically significant. (p=0.213). At 8 weeks duration the Serum Vit-D level was 62.419 in the test group and in control it was 37.788, the difference is statistically significant. (p<0.0001) and at 12 weeks also the change is significant. (p<0.0001). Conclusion: The rate of seroconversion was faster among Vitamin D group. Vitamin D supplementation can safely and efficiently raise the proportion of sputum smear and culture conversion. However, it may not have enough positive impacts on the time to sputum conversion. The participants who received vitamin D saw a speedier improvement in quality of life and a quicker reduction in the intensity of TB-related symptoms.