The aim of study is provide basic data about the histological features of the liver of two species of birds, which differ in their classification, habitat, nutrition and activity. The birds were omnivorous Black francolin(francolinus) and carnivorous Barn Owl (Tyto alba). To make this purpose a 10 mature healthy males birds were bought from the local suppliers at common markets at Al-Samawa city .(5 birds from each species) , all birds were anesthetized and dissected directly. The results of histological study, our results explained that the liver in the two species of birds were surrounded by a thin layer of dense connective tissue called Glissons capsule, this capsule different in size in owl and francolin thick layer in francolin than of owl . The hepatic parenchyma in studied birds were not clearly identified divided into distinct lobules due to absence of indiscernible hepatic connective tissue septa. The basic unit of the parenchyma of liver is hepatocyte which arranged in radially around the central vein as a hepatocords consists from two – several cell in thickness in barn owl, but in francolin bird, hepatic cells are composed of chains of hepatic cell. The lobules surrounded by portal area peripherally which made from dense connective tissues , it constitutes of portal vein, hepatic artery, and bile duct, in addition to lymphatic vessels which lining by endothelium.The histochemical study showed formed differ size of glycogen granules in cytoplasm of hepatocytes and The glycogen appears as pink-color deposits as identified throughout the cellular parenchyma of the liver especially in francolin,while glycogen appears less frequent in owl.