Background: To evaluate serum D-Dimer levels in carcinoma breast patients. Materials and Methods: In this prospective comparative hospital-based study, 65 diagnosed cases of operable breast carcinoma not started on any treatment for cancer and 65 healthy women were included as cases and controls, respectively. Peripheral venous blood was analyzed for D-dimers detected by Quantitative immune-turbidimetric latex assay. Results: It was observed that D-dimer levels significantly correlated with clinical stage (r=0.583; p<0.05), histological grade (r=0.655; p<0.05), ER/PR receptor (r=0.534; p<0.05), lymph node status (r=0.471; p<0.05) and presence of lymphovascular invasion (r=0.507; p<0.05). Conclusion: D-dimer is a simple, non-invasive, quick laboratory investigation that may be considered a good indicator for determining the clinical stage, progression of disease and lymphovascular invasion.