By increasing the percentage of morphologically normal sperm (MNS) and active sperm motility, pentoxifylline (PX) and caffeine (CF) have been demonstrated to play a significant effect in in vitro sperm activation. This, in turn, impacts the success of fertilization potential. Objective: In order to better understand the impact of activated sperm on the rate of pregnancy after intrauterine insemination, the current study aims to investigate the effects of combining CF alone or in combination with PX with the medium designed for in vitro sperm activation (Ham's F-12) on specific sperm function parameters in the oligoasthenoteratozoospermic patients. Subjects, Material and methods: The three groups based on the sperm stimulants utilized for the intrauterine insemination procedures that came after the in vitro sperm activation. 40 couples made up group one, which included CF-treated semen samples (1 ml added to Ham's F-12 culture medium); 25 couples made up group two, which included CF+PX-treated semen samples (1 ml added to Ham's F-12 culture medium; and 30 couples made up group three, which included Ham's F-12 culture medium as a control group. Results: After in vitro sperm activation, A very notable increase was obseved in active sperm motility and MNS percentages in oligoasthenoteratozoospermic male partners and when compared with before activation. In vitro sperm activation with CF- containing culture media had significantly increased the pregnancy rate following IUI in comparison with CF+PX treated media (30% versus 20%). Conclusion: The culture media supplemented with CF and CF+PX are excellent choices for the in vitro sperm activation method. Compared to other treated culture media, adding CF to culture media for in vitro sperm activation was linked to a greater pregnancy rate after IUI.