Abstract: Background- The main goal in periodontal surgery is the healing by primary intention because there is less scarring, more rapid healing and reduced discomfort. Cyanoacrylate is a tissue adhesive which provides benefits to the patient as a faster healing and less postoperative pain. Flap surgery for periodontal reattachment, demands close postoperative adaptation for the gingival tissue onto the prepared tooth surface. Objective- To evaluate the healing after periodontal flap surgery using isoamyl 2‑ cyanoacrylate and silk sutures. Material and Methods- In this study, 40 patients of systemically healthy will be selected. Groups will be divided into test and control which consist of 20 defects in each. Informed written consent will be obtained from all the subjects before the surgery. Clinical measurements like Plaque index, Papillary bleeding index, Probing pocket depth, Healing index will be recorded. The test group will be treated by isoamyl 2‑ cyanoacrylate, while the control group will be treated by Silk suture after periodontal flap surgery. Patients will be recalled at 1, 3, 6 months post surgically for periodontal re-evaluation. Expected Result- When all the parameters will be compared at 6 months post-operatively to baseline data, both the treatment group (test and control) will show significant CAL gain, PPD reduction. Test group will be found better healing as compared to control. Conclusion- Both the treatment group (test and control) will show significant CAL gain, PPD reduction. This study will be achieved effective healing response after periodontal flap surgery with sutures as compared to cyanoacrylate