Background: Dentine hypersensitivity is a common complaint among dental patients. Recently, a novel stabilized calcium sucrose phosphate dentifrice was introduced that offers a desensitizing benefit. The primary aim of this study was to compare the in vivo efficacy of this novel dentifrice with Calcium sodium phosphosilicate and a non-desensitizing dentifrice, on dentin hypersensitivity in a four-week period.